Lift a universal of sterile nutrient broth in the left hand.
Remove the lid of the universal with the little finger of the right hand which still holds the charged loop or pipette. Do not put down the lid.
Flame the neck of the universal.
Insert the loop charged with inoculum into the sterile broth. Touch on the inside of the universal and withdraw.
If using a pipette, gently release the required number of drops from the pipette into the culture and withdraw pipette.Do not agitate or cause bubbles in the liquid medium.Flame the neck of the universal, replace lid and place the universal on the bench.
Flame the loop and place on heat resistant mat/ place pipette in discard jar.
Tighten lid of universal to make secure but do not overtighten.
Partially lift the lid of the Petri dish containing the sterile malt agar medium.
Place the cube/bore of agar or fungal mycelium on to the centre of the sterile agar. Use a mounted needle which has been flamed in ethanol to assist with the transfer process if necessary.
Dip the implement in ethanol, flame as above and place on heat resistant mat.
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