- Taking care not to touch the cotton wool end, remove a sterile swab from the pack and hold it in your right hand.
- Lift a bottle of sterile water in the left hand. Do not put down swab.
- Remove the lid of the bottle with the little finger of the right hand which is still holding the swab.
- Flame the neck of the bottle.
- Insert and withdraw the cotton wool bud.
- Flame the neck of the bottle and replace the lid.
- Rub the swab on the area to be tested for 10 – 15 seconds.
- Loosen the top of the bottle containing the slope culture so that it can be removed easily.
- Hold the loop in the right hand.
- Flame the loop and allow to cool. Do not put down loop or wave it around.
- Lift the universal containing the inoculum with the left hand.
- Remove the lid of the universal with the little finger of the right hand. (Turn the bottle, not the lid). Do not put down the lid.
- Flame the neck of the universal.
- Bringing the bottle to the loop, insert the loop into the bottle, touch a small area of growth and withdraw loop.
- At all times, hold the loop as still as possible.
- Flame the neck of the universal.
- Replace the lid on the universal using the little finger, turning the bottle not the lid.
- Place slope on bench.
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- Hold the loop in the right hand.
- Flame the loop and allow to cool. Do not put down loop.
- With the left hand, lift the lid a little of the Petri dish containing the inoculum.
- Touch a single colony with the wire loop.
- Withdraw loop carefully without touching plate. Do not put down loop or wave it around.
- Replace lid of Petri dish.
- Loosen the tops of the universals containing the broth cultures so that they can be removed easily.
- Remove the pipette from its sterile container taking care not to touch the tip, attach bulb and hold in the right hand.
- Lift the universal containing the inoculum with the left hand.
- Remove the lid of the universal with the little finger of the right hand. (Turn the bottle, not the lid). Do not put down the lid.
- Flame the neck of the universal.
- Squeeze the teat of the pipette before it enters the broth so that it does not cause bubbles and possible aerosols and withdraw a little of the culture.
- At all times, hold the pipette as still as possible.
- Flame the neck of the universal.
- Replace the lid on the universal using the little finger, turning the bottle not the lid.
- Place universal on bench.
Take care that the culture does not drip from the pipette. Work over paper towels moistened with disinfectant if working with novices.
- Dip the implement in ethanol, place in Bunsen flame briefly and allow ethanol to burn off. Do not put down.
- Lift the lid a little of the Petri dish containing the inoculum with the left hand.
- Hold the inoculating implement in the right hand.
- Keep the ethanol at a safe distance from the Bunsen flame.
- Lift a cube, strands or core of agar with fungal mycelium.
- Withdraw implement from Petri dish.
- Replace lid of Petri dish.
- At all times, hold the inoculating implement as still as possible.